Association of germline variants with KRAS-mutation status in colorectal cancer.
Abstract
Somatic mutations in KRAS are a common driver of colorectal cancer (CRC) and present at different frequencies by race, sex, tumor site, ethnicity, and genetic similarity. Inherited germline variants may influence tumor somatic mutation frequency by altering mutation or DNA repair processes or altering cellular, immunological and/or microenvironmental responses after a mutation. We hypothesized that the germline genetic background modifies somatic KRAS mutation frequency in CRC. To test this, we performed a genome-wide association study (GWAS) in 7071 individuals with CRC, using KRAS mutation status as the phenotype. Single-nucleotide variants were chosen for validation analyses based on P values from the discovery GWAS, predicted in silico functional effects, and proximity to genes with potential cancer relevance. A validation analysis of 101 SNVs of interest was performed in 2482 individuals. No SNVs were significantly associated with KRAS-mutant CRC (P value < 0.0005). One variant rs73067863-T showed a non-significant exploratory association with fewer KRAS-mutant tumors in the combined sample (P value = 9.7 × 10<sup>-7</sup>, OR = 0.75). Follow-up studies are needed to determine if these or other germline variants impact population differences in KRAS mutations in CRC.
EDRN PI Authors
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Medline Author List
- Aglago EK
- Berndt SI
- Bloomer A
- Brenner H
- Buchanan DD
- Campbell PT
- Cao Y
- Chan AT
- Cheng I
- Dauch C
- Dimou N
- Drew DA
- Figueiredo J
- French AJ
- Gandhi T
- Georgeson P
- Giannakis M
- Giles GG
- Gomez M
- Gruber SB
- Hampel H
- Hoffmeister M
- Huang WY
- Hullar MAJ
- Huyghe JR
- Loroña N
- McElroy JP
- Meadows O
- Moreno V
- Newton CC
- Nowak JA
- Obón-Santacana M
- Ogino S
- Pearlman R
- Pellatt A
- Peoples AR
- Permuth JB
- Peters U
- Phipps AI
- Ramroop J
- Schmit SL
- Schoen RE
- Siegel EM
- Steinfelder RS
- Stevens P
- Sun W
- Teer JK
- Thomas CE
- Tjader NP
- Toland AE
- Trinh QM
- Tsilidis K
- Ugai T
- Um CY
- Van Guelpen B
- Zaidi SH