Design and preliminary report of a randomized phase IIb clinical trial of multitargeted recombinant adenovirus 5 vaccines against CEA, MUC1, and brachyury (Tri-Ad5) and the IL-15 receptor superagonist nogapendekin alfa inbakicept in Lynch syndrome (TRIAD5

Abstract

Lynch syndrome (LS) is a hereditary cancer syndrome that increases risk for colorectal and other cancers. We hypothesize that vaccines against tumor-associated antigens CEA, MUC1, and brachyury, simultaneously delivered in an adenovirus serotype-5 vector (Tri-Ad5) combined with the immune-enhancing IL-15 receptor superagonist nogapendekin-alfa-inbakicept (NAI) will reduce the incidence of colorectal neoplasms in LS carriers.

In this ongoing phase IIB double-blind placebo-controlled trial, two open-label safety phases (SPs) assessed safety of Tri-Ad5 alone (SP1, n=10) and with added NAI (SP2, n=10). A randomized controlled trial (RCT) follows the SPs. After baseline colonoscopy, vaccine dosing occurs at weeks 0, 4 and 8; Tri-Ad5 alone in SP1 and Tri-Ad5 plus NAI in SP2, with identical boosters at Week 52. The RCT phase participants (n=138) were randomized to receive Tri-Ad5 plus NAI or placebo. All participants complete colonoscopy at Weeks 52 and 104 for assessment of the primary endpoint: cumulative colorectal neoplasm incidence. Secondary endpoints include safety, tolerability, and immunogenicity.

All 20 SP participants (median age 57.5 (range 42-75), 70% female, 15% minority) received all prime and booster series. SP1 participants reported 139 adverse events (AEs) and SP2 participants reported 178. AEs were predominantly grade 1; no treatment-related serious adverse events (SAEs) occurred. The most common treatment-related AEs were reactogenic events including grade 3 rash (without skin necrosis or breakdown) at NAI injection site (100% of SP2 participants). The RCT phase (n=138) recently completed accrual.

In LS carriers without active cancer, the combination of Tri-Ad5 + NAI was well-tolerated; the RCT phase is ongoing.

https://clinicaltrials.gov/study/, identifier NCT05419011.

EDRN PI Authors
Medline Author List
  • Aquila L
  • Ballestar V
  • Bansal A
  • Barroilhet LM
  • Bauman J
  • Benante KA
  • Bengtson L
  • Boardman LA
  • Brenner D
  • Burke CA
  • Della'Zanna G
  • Djuric Z
  • Donahue RN
  • Eickhoff J
  • Gabitzsch E
  • Gulley JL
  • Hall MJ
  • Horn LA
  • Hurley KE
  • Hyun M
  • Khan SA
  • Lim R
  • Madan R
  • Moore DF
  • Muller C
  • Palena C
  • Patel SG
  • Richmond E
  • Samaddar S
  • Samadder NJ
  • Schlom J
  • Schuetze S
  • Scott AJ
  • Sender L
  • Sharma P
  • Singh A
  • Soon-Shiong P
  • Stanich PP
  • Stoffel EM
  • Szabo E
  • Thompson P
  • Toney NJ
  • Umar A
  • Vilar E
  • Xu Y
  • Zaborek J
PubMed ID
Appears In
Front Immunol, 2026 None (issue None)