3D pathology-guided microdissection.
Abstract
Traditional micro- and macrodissection techniques enable the extraction of localized regions in thin tissue sections for molecular analysis. Despite the growing use of three-dimensional (3D) microscopy, analogous methods for volumetric microdissection are lacking. Here we have developed a 3D microdissection method based on computer numerical controlled milling integrated with open-top light-sheet microscopy. We demonstrate the ability to study tumor evolution along convoluted 3D branching architectures, which is inaccessible to two-dimensional methods.
EDRN PI Authors
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Medline Author List
- Baraznenok E
- Bishop KW
- Brenes D
- Figiel S
- Gao G
- Grady WM
- Haffner MC
- Hamdy FC
- Han Q
- Hsieh HC
- Konnick EQ
- Liu JTC
- Mills IG
- Paulson TG
- Pritchard CC
- Rao SR
- Reddi DM
- Reder NP
- Serafin R
- True LD
- Valk JE
- Wang R
- Woodcock DJ
- Yan R