Abbreviated Name

PTM Microarray

Lead Investigator

Zangar, Richard (Rick) C. — Pacific Northwest National Laboratory

Coordinating Investigator

Zangar, Richard (Rick) C. — Pacific Northwest National Laboratory

Involved Investigators

• Marks, Jeffrey — Duke University Medical Center
• Zangar, Richard (Rick) C. — Pacific Northwest National Laboratory

Abstract

No abstract availalbe.

Aims

Aim 1) Develop antibody microarray chips that can be used to measure PTMs associated with glycosylation state or nTyr- or HNE-adducts. This chip will target abundant proteins we have identified in NAF that are also found in the circulation. Aim 2) Evaluate two sets of human plasma samples from women with and without breast cancer using the chips developed in Aim 1. The first set of samples is from the Clinical Breast Care Program (CBCP, samples collected at Walter Reed Army Medical Center) and contains 80 samples from control women or women with DCIS or stage 1 or 2 breast cancer. This set of samples will determine if these assays can detect early disease. The second set of samples will be from Dr. Jeff Marks (Duke University) and will contain 60 samples evenly split between three study groups: control women and women with Her2+/ER- tumor, and women with ER+/Her2- breast cancer. This set of samples will determine if these assays can detect both early- and late-stage disease as well as differentiate between different subtypes of cancer.

Analytic Method

ELISA microarray

Publications

• No publications available at this time for this protocol.

Biomarkers

• No biomarkers available at this time for this protocol.

Data Collections

• No data collections available at this time for this protocol.

Start Date

Dec 31 2007

Estimated Finish Date

Dec 30 2008

Finish Date

Dec 30 2008

Protocol ID

233

Protocol Type

Collaboration

Fields of Research

• Proteomics

Collaborative Group

Breast and Gynecologic Cancers Research Group

Cancer Types

• Malignant neoplasm of breast