Biomarker Detection Using NAPPA Tumor Antigen Arrays: EDRN Supplement

Abbreviated Name
Biomarker Detection Using NAPPA Tumor Antigen Arrays: EDRN Supplement
Lead Investigator
Anderson, Karen — Arizona State University
Coordinating Investigator
No coordinating investigator
Involved Investigators

Abstract

No abstract availalbe.

Aims

1.B. Project Objectives Aim 1: Generation of NAPPA protein microarrays expressing the fifty most common p53 point mutations for solid tumors as well as deletion constructs of p53. Aim 2: Identify p53 mutation-specific antibodies in a test set of breast, prostate, and ovarian cancer patient sera. a.   Using test sera from patients with prostate, ovarian, and breast cancers, identify patient sera that contain anti-p53 antibodies. b.   Using p53-antibody-positive sera from patients, screen the p53 mutation microarray to identify mutation-specific and domain-specific antibodies c.   Using p53-antibody-negative sera from patients, screen the p53 mutation microarray to identify mutation-specific antibodies d.   Determine the sensitivity and specificity of individual antibodies that distinguish patient sera from normal sera. e.   Determine if mutation-specific antibodies can distinguish tumor types.

Analytic Method

Various including quantile regression, Fishers exact, binomial proportion and others. The methods are not yet fixed because we are still in discovery.

Publications

  • No publications available at this time for this protocol.

Biomarkers

Data Collections

  • No data collections available at this time for this protocol.
Start Date
Jan 2 2008
Estimated Finish Date
Dec 31 2008
Finish Date
Dec 31 2008
Protocol ID
190
Protocol Type
Collaboration
Fields of Research
  • Proteomics
Collaborative Group
Breast and Gynecologic Cancers Research Group
Cancer Types
  • Malignant neoplasm of breast
  • Malignant neoplasm of ovary
  • Malignant neoplasm of prostate

Associated Forms