Abbreviated Name

SELDI -I

Lead Investigator

Semmes, John — Eastern Virginia Medical School

Coordinating Investigator

Zheng, Yingye — Fred Hutchinson Cancer Center

Involved Investigators

• Chesnut, Gregory — Center for Prostate Disease Research Uniformed Services University of the Health Sciences and the Walter Reed National Military Medical Center
• Semmes, John — Eastern Virginia Medical School
• — Johns Hopkins University Department of Urology
• Leach, Robin J — University of Texas Health Science Center at San Antonio
• Srivastava, Sudhir — National Cancer Institute
• Bigbee, William L. — University of Pittsburgh Cancer Institute
• Zhao, Yingming — UT Southwestern Medical Center
• Grizzle, William E. — University of Alabama at Birmingham
• Adam, Bao-Ling — Medical College of Georgia
• Zheng, Yingye — Fred Hutchinson Cancer Center

Abstract

see objectives

Aims

Specific Aim IA/Phase IA - The EDRN-Prostate-SELDI Investigational Collaboration (EPSIC) will implement a process to synchronize SELDI output at the seven participating institutions using a single source of pooled normal sera (QC). Specific Aim IB/Phase IB - The EPSIC will synchronize SELDI output at the seven participating institutions using prostate cancer/control samples with verified presence of optimized diagnostic peaks. The reproducibility of QSTAR will also be determined using the same samples and compared to that of the PBS-II data. Specific Aim IC/Phase IC - The EPSIC will implement a seven-center collaboration to synchronize SELDI output using a new and expanded cohort of prostate cancer cases and controls obtained from all sites. The reproducibility of QSTAR will also be determined using the same samples and compared to that of the PBS-II data.

Analytic Method

The criteria for agreement among labs is based on the agreement in mass location and agreement in protein intensity. Since the true protein mass is unknown at each protein mass measured from the machine (there is always some measurement error, no matter how small it is), the mass location agreement is determined by the agreement of the locations of peaks between each lab and the reference lab (EVMS) and between each lab and other four labs combined. More specifically, after peak identification and alignment are performed by each lab, the coefficient of variation (CV) and intra-class correlation coefficient (ICC) for peak location measures and for peak intensity measures will be calculated for each peak location. The between-lab variation will be compared to the between-sample variation.

Outcome

see objectives

Publications

• Enhancement of sensitivity and resolution of surface-enhanced laser desorption/ionization time-of-flight mass spectrometric records for serum peptides using time-series analysis techniques.

Biomarkers

• Protein mass location

Data Collections

• EVMS Mass Spec Data

Start Date

Dec 23 2002

Estimated Finish Date

Dec 23 2003

Finish Date

Feb 1 2005

Protocol ID

109

Protocol Type

Validation

Fields of Research

• Other

Collaborative Group

Prostate and Urologic Cancers Research Group

Cancer Types

• Malignant neoplasm of prostate

Phased Status

1