EDRN-WHI Pre-Clinical Colon Ca Specimens

Abbreviated Name
EDRN/WHI COLON
Lead Investigator
Hanash, Samir — The University of Texas MD Anderson Cancer Center
Coordinating Investigator
Zheng, Yingye — Fred Hutchinson Cancer Center
Involved Investigators

Abstract

A pioneering collective discovery project using colon cancer pre-diagnostic plasma and controls is proposed as a joint Early Detection Research Network-Women’s Health Initiative (EDRN-WHI) study. One half ml of plasma from 100 colon cancer cases and an equal number of pair matched controls from the Observational Study (OS) will be aliquotted and made available by WHI for analysis using complementary proteomic platforms to assess the potential of proteomics technologies to generate leads concerning early detection markers or risk prediction markers for colon cancer, and to compare and contrast findings between proteomic platforms. Colon cancer cases that occur within a span of 18 months following year-3 OS blood draw will be selected. The platforms considered will include the commonly used ‘peptide shotgun’ approach, a comprehensive, quantitative intact plasma protein approach, tumor antigen/autoantibody approaches, a peptide capture mass spectrometry approach, and glycoprotein enrichment approaches. In phase 1, which is expected to last six months, participating investigators will analyze their own data. However by the end of phase one investigators are expected to have deposited their data in a central database for integrated analysis. The data will be publicly accessible following publication. The NCI Early Detection Research Network is expected to contribute most of the funding needed for this project.

Aims

Specific Aims are identified by each assay laboratory.

Analytic Method

The primary analysis for this study is to identify proteins/peptides that have potential as colon cancer early detection or risk prediction markers for subsequent validation. The secondary aim is to see if some proteins/peptides are complementary in their early detection abilities so their combinations will improve their sensitivity and specificity. Since thousands of protein/peptides will be identified and/or quantified, one important issue is to quantify and control the False Discovery Rate (# of false positive finding / # of selected markers as differentially expressed) and maintain reasonable power to detect truly differentially expressed markers. Another important feature of the data analysis is that the derived data from mass spectrometry critically depends on the algorithms of identifying the peptides, assigning protein identities and intensities. Coordinating center biostatisticians need work closely with proteomics bioinformaticians and computational biologists with expected iterations between data processing and data analyses.

Outcome

A pioneering collective discovery project using colon cancer pre-diagnostic plasma and controls is proposed as a joint Early Detection Research Network-Women’s Health Initiative (EDRN-WHI) study. One half ml of plasma from 100 colon cancer cases and an equal number of pair matched controls from the Observational Study (OS) will be aliquotted and made available by WHI for analysis using complementary proteomic platforms to assess the potential of proteomics technologies to generate leads concerning early detection markers or risk prediction markers for colon cancer, and to compare and contrast findings between proteomic platforms. Colon cancer cases that occur within a span of 18 months following year-3 OS blood draw will be selected. The platforms considered will include the commonly used ‘peptide shotgun’ approach, a comprehensive, quantitative intact plasma protein approach, tumor antigen/autoantibody approaches, a peptide capture mass spectrometry approach, and glycoprotein enrichment approaches. In phase 1, which is expected to last six months, participating investigators will analyze their own data. However by the end of phase one investigators are expected to have deposited their data in a central database for integrated analysis. The data will be publicly accessible following publication. The NCI Early Detection Research Network is expected to contribute most of the funding needed for this project.

Publications

Biomarkers

  • No biomarkers available at this time for this protocol.

Data Collections

Start Date
Jan 17 2006
Estimated Finish Date
Jan 17 2007
Finish Date
Jan 17 2008
Protocol ID
126
Protocol Type
Collaboration
Fields of Research
  • Glycomics
  • Proteomics
Collaborative Group
G.I. and Other Associated Cancers Research Group
Cancer Types
  • Malignant neoplasm of colon

Associated Forms