MSA Bladder Reference Set Application: Charles Rosser-Hawaii (2014)

Abbreviated Name
MSA Bladder Ref Set App: Rosser (2014)
Lead Investigator
Rosser, Charles J — University of Hawaii Cancer Center
Coordinating Investigator
Zheng, Yingye — Fred Hutchinson Cancer Center
Involved Investigators

Abstract

No abstract availalbe.

Aims

Discovery cohort - 200 DNA samples from exfoliated cells (100 BCa and 100 controls) from a reference set at EDRN will be screened via direct PCR and sequencing for mutations with PAI-1 (promoter and exons 1-9) and ANG (promoter and exons 1-3).

Analytic Method

Mutational analysis will be assessed using Ion Reporter™ Software located in our Genomic Shared Resource. Fisher’s exact test will be used to assess the association of PAI-1 and ANG genetic alterations with clinicopathologic factors or mutations with each other. A P-value of < 0.05 will be considered to be statistically significant.

Comments

No comments available.

Outcome

PAI1 exon 9 shows marked differences between cancer and no cancer, both when looking at whether there is any polymorphism present (64% of cancers and 35% if no cancers) or when looking at the number of polymorphisms an individual has (of those with a polymorphism, those with cancers had a mean of 2.14 polymorphisms, those without cancer had a mean of 1.88). The other sites examined did not show statistically significant differences between cancer and no cancer. I've not yet looked at the type of polymorphism or the different types of non-cancer controls we have in the set.

Secure Outcome

No secure outcome available.

Publications

Biomarkers

Data Collections

  • No data collections available at this time for this protocol.
 Team Project
Finish Date
Jul 21 2015
Protocol ID
394
Protocol Type
Reference Set
Fields of Research
  • Genomics
  • Proteomics
Collaborative Group
Prostate and Urologic Cancers Research Group
Cancer Types
  • Malignant neoplasm of bladder

Associated Forms